Location
LSU Health Sciences Center - New Orleans
Event Website
https://digitalscholar.lsuhsc.edu/surgery_s/
Document Type
Event
Start Date
4-5-2023 10:30 AM
End Date
4-5-2023 10:40 AM
Description
Objectives: The dynamic environment of the peritoneal cavity has not been well-studied in the trauma patient population, which is high risk for inflammatory dysregulation given the systemic changes induced by traumatic injury. The objective of this study is to better characterize the milieu of the peritoneal cavity to understand the mechanisms that lead to infectious complications which impair the healing of damage tissue.
Methods: Peritoneal fluid was collected from adult trauma patients who underwent damage control laparotomy during one of their subsequent return trips to the operating room for re- exploration (N=4). Cytokine profiles were analyzed and determined using a Multiplex assay. The fluid was additionally cultured on tryptic salt and MacConkey’s agar plates to determine the presence of bacteria.
Results: A total of 4 trauma patients were enrolled. Bowel injuries were found in 3 of the patients upon initial laparotomy (Injury Group), and 1 patient had a laparotomy that was negative for injury (Control). The injury group had higher levels of IL-4, IL-6, IL-10, and IL-8. Complications noted in the injury group included ECF (n=1) and NSTI (n=1). IL-6 was not detectable in the patient with an ECF. Microbial cultures grew organisms (gram positive, gram negative, and anaerobes) in 2 of the patients from the injury group. No microbial growth noted from the control patient.
Conclusions: This is a pilot study with on-going studies in process to increase sample size and refine experimental approach. Gaining a more sophisticated understanding of the local inflammatory response to abdominal trauma can provide valuable insight into therapeutic strategies to mitigate the impacts of a dysfunctional cytokine and resultant post-operative complications in trauma patients.
Recommended Citation
Deville, Paige; Dennis, Jenna; Fontenot, C.; Warren, O.; Carter, Jeff; Phelan, Herb; Schoen, Johnathan; Hobden, Jeffery; and Smith, Alison A., "Determining the Inflammatory and Microbial Profiles of Peritoneal Fluid from Trauma Patients Following Damage Control Laparotomy" (2023). Surgery Research Symposium. 14.
https://digitalscholar.lsuhsc.edu/surgery_s/2023/postera/14
Included in
Determining the Inflammatory and Microbial Profiles of Peritoneal Fluid from Trauma Patients Following Damage Control Laparotomy
LSU Health Sciences Center - New Orleans
Objectives: The dynamic environment of the peritoneal cavity has not been well-studied in the trauma patient population, which is high risk for inflammatory dysregulation given the systemic changes induced by traumatic injury. The objective of this study is to better characterize the milieu of the peritoneal cavity to understand the mechanisms that lead to infectious complications which impair the healing of damage tissue.
Methods: Peritoneal fluid was collected from adult trauma patients who underwent damage control laparotomy during one of their subsequent return trips to the operating room for re- exploration (N=4). Cytokine profiles were analyzed and determined using a Multiplex assay. The fluid was additionally cultured on tryptic salt and MacConkey’s agar plates to determine the presence of bacteria.
Results: A total of 4 trauma patients were enrolled. Bowel injuries were found in 3 of the patients upon initial laparotomy (Injury Group), and 1 patient had a laparotomy that was negative for injury (Control). The injury group had higher levels of IL-4, IL-6, IL-10, and IL-8. Complications noted in the injury group included ECF (n=1) and NSTI (n=1). IL-6 was not detectable in the patient with an ECF. Microbial cultures grew organisms (gram positive, gram negative, and anaerobes) in 2 of the patients from the injury group. No microbial growth noted from the control patient.
Conclusions: This is a pilot study with on-going studies in process to increase sample size and refine experimental approach. Gaining a more sophisticated understanding of the local inflammatory response to abdominal trauma can provide valuable insight into therapeutic strategies to mitigate the impacts of a dysfunctional cytokine and resultant post-operative complications in trauma patients.
https://digitalscholar.lsuhsc.edu/surgery_s/2023/postera/14