Document Type
Article
Publication Date
5-1-2020
Publication Title
Nature Communications
Abstract
Optogenetic genome engineering tools enable spatiotemporal control of gene expression and provide new insight into biological function. Here, we report the new version of genetically encoded photoactivatable (PA) Cre recombinase, PA-Cre 3.0. To improve PA-Cre technology, we compare light-dimerization tools and optimize for mammalian expression using a CAG promoter, Magnets, and 2A self-cleaving peptide. To prevent background recombination caused by the high sequence similarity in the dimerization domains, we modify the codons for mouse gene targeting and viral production. Overall, these modifications significantly reduce dark leak activity and improve blue-light induction developing our new version, PA-Cre 3.0. As a resource, we have generated and validated AAV-PA-Cre 3.0 as well as two mouse lines that can conditionally express PA-Cre 3.0. Together these new tools will facilitate further biological and biomedical research.
PubMed ID
32358538
Volume
11
Issue
1
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Recommended Citation
Morikawa, Kumi; Furuhashi, Kazuhiro; de Sena-Tomas, Carmen; Garcia-Garcia, Alvaro L.; Bekdash, Ramsey; Klein, Alison D.; Gallerani, Nicholas; Yamamoto, Hannah E.; Park, Seon Hye E.; Collins, Grant S.; Kawano, Fuun; Sato, Moritoshi; Lin, Chyuan Sheng; Targoff, Kimara L.; Au, Edmund; Salling, Michael C.; and Yazawa, Masayuki, "Photoactivatable Cre recombinase 3.0 for in vivo mouse applications" (2020). School of Medicine Faculty Publications. 1547.
https://digitalscholar.lsuhsc.edu/som_facpubs/1547
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