ROLE OF micro RNA-125b (miRNA-125b) IN THE HUMAN BRAIN -FUNCTIONS IN ASTROGLIOSIS AND GLIAL CELL PROLIFERATION
Document Type
Presentation
Start Date
22-10-2010 10:45 AM
End Date
22-10-2010 12:00 PM
Description
Purpose of Study: Micro RNAs (miRNAs) are emerging as important post-transcriptional regulators of gene expression in the brain. Certain miRNA species, including miRNA-125b, are highly enriched in aging human brain cells and tissues, in Alzheimer's disease (AD), Down's Syndrome (DS), and in glioblastoma multiforme (GBM). Here we analyzed prominent brain cell types of the neurovascular unit -neurons, glia and endothelial cells in primary culture -for miRNA-125b abundance during oxidative stress, brain cell growth and aging.
Methods Used: Normal human astroglial (HAG) primary culture; human neuronal-glial (HNG) primary co-culture; human brain microvessel endothelial cell (hBMEC) primary culture; human brain tissues; micro RNA array; DNA array; Northern analysis, RT-PCR, Western analysis; ELISA; bioinformatics, statistical analysis.
Summary of Results: In contrast to HNG and hBMEC primary cell cultures, miRNA-125b was found to be highly abundant in HAG cells, and especially so in actively proliferating HAG cell cultures.
Conclusions: We report that the levels of miRNA-125b are significantly up-regulated in actively dividing HAG cells, suggesting that miRNA-125b plays some role in HAG cell proliferation or astrogliosis. The results further suggest that miRNA-125b up-regulation strongly associates with astroglial cell proliferation that is a characteristic feature of several human neurological disorders including AD, DS and GBM.
Support: These studies were supported by an Alzheimer Association Investigator-Initiated Research Grant IIRG-09-131729 (WJL).
Recommended Citation
Bhattacharjee, Surjyadipta; Cui, J. G.; Culicchia, F.; and Bazan, N. G., "ROLE OF micro RNA-125b (miRNA-125b) IN THE HUMAN BRAIN -FUNCTIONS IN ASTROGLIOSIS AND GLIAL CELL PROLIFERATION" (2010). Dr. Joseph M. Moerschbaecher, III Graduate Research Day. 4.
https://digitalscholar.lsuhsc.edu/grad_rs/2010/poster1/4
ROLE OF micro RNA-125b (miRNA-125b) IN THE HUMAN BRAIN -FUNCTIONS IN ASTROGLIOSIS AND GLIAL CELL PROLIFERATION
Purpose of Study: Micro RNAs (miRNAs) are emerging as important post-transcriptional regulators of gene expression in the brain. Certain miRNA species, including miRNA-125b, are highly enriched in aging human brain cells and tissues, in Alzheimer's disease (AD), Down's Syndrome (DS), and in glioblastoma multiforme (GBM). Here we analyzed prominent brain cell types of the neurovascular unit -neurons, glia and endothelial cells in primary culture -for miRNA-125b abundance during oxidative stress, brain cell growth and aging.
Methods Used: Normal human astroglial (HAG) primary culture; human neuronal-glial (HNG) primary co-culture; human brain microvessel endothelial cell (hBMEC) primary culture; human brain tissues; micro RNA array; DNA array; Northern analysis, RT-PCR, Western analysis; ELISA; bioinformatics, statistical analysis.
Summary of Results: In contrast to HNG and hBMEC primary cell cultures, miRNA-125b was found to be highly abundant in HAG cells, and especially so in actively proliferating HAG cell cultures.
Conclusions: We report that the levels of miRNA-125b are significantly up-regulated in actively dividing HAG cells, suggesting that miRNA-125b plays some role in HAG cell proliferation or astrogliosis. The results further suggest that miRNA-125b up-regulation strongly associates with astroglial cell proliferation that is a characteristic feature of several human neurological disorders including AD, DS and GBM.
Support: These studies were supported by an Alzheimer Association Investigator-Initiated Research Grant IIRG-09-131729 (WJL).