SPATIAL CORRELATION OF MOUSE PHOTORECEPTOR-RETINAL PIGMENT EPITHEIUM THICKNESS BETWEEN SPECTRAL DOMAIN OCULAR COHERENCE TOMOGRAPHY AND HISTOLOGY.
Document Type
Presentation
Start Date
22-10-2010 10:45 AM
End Date
22-10-2010 12:00 PM
Description
Introduction: Classic evaluation of retinal degeneration relies on histological sample preparation and light microscopy, requiring extensive animal numbers, costly embedding materials, and many technician hours. Optical Coherence Tomography (OCT) is a new technology that will greatly reduce preparation time, cost, and allow longitudinal analysis of single animals throughout experimental protocols. Recently, OCT scans from a commercially available imaging system have yielded retinal thickness values comparable to histology. However, these measurements are based on single point analysis of images. Therefore, the purpose of this study was to determine if mouse linear expanses of photoreceptor cell loss in light-induced retinal degeneration could be accurately characterized by OCT (Spectralis, Heidelberg Engineering).
Methods: Balb/c mice (25g) were acclimated for 7 days, anesthetized and imaged via SD-OCT. Following 2 days recovery, eyes were dilated and mice stimulated with light (4 kLx, Oh and 6h). After 8 h of dark, 10 days cycled light, and imaged with SD-OCT; eyes were collected for plastic sections, from which the outer nuclear layer (ONL) thickness could be measured. OCT photoreceptor integrity was measured using Heidelberg Engineering software and raw data of entire retinal expanses accessed via custom imageJ plugin. Histologic sections of eyes were measured optically using Nikon NIS software. OCT and histological measurements were compared at 25 μm increments along the superior-inferior meridian.
Results: Here we report that using the Spectralis, retinal thickness of linear expanses of OCT data can be accurately assessed using a custom lmageJ plugin. Moreover, mouse retinal layer thickness values obtained with this plug-in exhibit a high correlation (R2=0.9042) to thickness measurements from histology of the same retinas. Conclusions: Our results show that OCT imaging of retinas can be used to achieve measurements of photoreceptor layer thickness that are comparable to those obtained from optical analysis of light microscopy. This indicates that a single animal can be accurately and repeatedly imaged by OCT throughout an experiment. Therefore, OCT is a reliable tool to evaluate time-relevant changes in retina.
Recommended Citation
Knott, Eric J., "SPATIAL CORRELATION OF MOUSE PHOTORECEPTOR-RETINAL PIGMENT EPITHEIUM THICKNESS BETWEEN SPECTRAL DOMAIN OCULAR COHERENCE TOMOGRAPHY AND HISTOLOGY." (2010). Dr. Joseph M. Moerschbaecher, III Graduate Research Day. 16.
https://digitalscholar.lsuhsc.edu/grad_rs/2010/poster1/16
SPATIAL CORRELATION OF MOUSE PHOTORECEPTOR-RETINAL PIGMENT EPITHEIUM THICKNESS BETWEEN SPECTRAL DOMAIN OCULAR COHERENCE TOMOGRAPHY AND HISTOLOGY.
Introduction: Classic evaluation of retinal degeneration relies on histological sample preparation and light microscopy, requiring extensive animal numbers, costly embedding materials, and many technician hours. Optical Coherence Tomography (OCT) is a new technology that will greatly reduce preparation time, cost, and allow longitudinal analysis of single animals throughout experimental protocols. Recently, OCT scans from a commercially available imaging system have yielded retinal thickness values comparable to histology. However, these measurements are based on single point analysis of images. Therefore, the purpose of this study was to determine if mouse linear expanses of photoreceptor cell loss in light-induced retinal degeneration could be accurately characterized by OCT (Spectralis, Heidelberg Engineering).
Methods: Balb/c mice (25g) were acclimated for 7 days, anesthetized and imaged via SD-OCT. Following 2 days recovery, eyes were dilated and mice stimulated with light (4 kLx, Oh and 6h). After 8 h of dark, 10 days cycled light, and imaged with SD-OCT; eyes were collected for plastic sections, from which the outer nuclear layer (ONL) thickness could be measured. OCT photoreceptor integrity was measured using Heidelberg Engineering software and raw data of entire retinal expanses accessed via custom imageJ plugin. Histologic sections of eyes were measured optically using Nikon NIS software. OCT and histological measurements were compared at 25 μm increments along the superior-inferior meridian.
Results: Here we report that using the Spectralis, retinal thickness of linear expanses of OCT data can be accurately assessed using a custom lmageJ plugin. Moreover, mouse retinal layer thickness values obtained with this plug-in exhibit a high correlation (R2=0.9042) to thickness measurements from histology of the same retinas. Conclusions: Our results show that OCT imaging of retinas can be used to achieve measurements of photoreceptor layer thickness that are comparable to those obtained from optical analysis of light microscopy. This indicates that a single animal can be accurately and repeatedly imaged by OCT throughout an experiment. Therefore, OCT is a reliable tool to evaluate time-relevant changes in retina.