Datasets: Increased Expression of Zip4 (Slc39a4) May also Occur in Many Different Cancers

Authors

Benjamin P. Weaver, University of Kansas
Yuxia Zhang, University of Utah
Stephen Hiscox, Cardiff University, UK
Grace L. Guo, University of Kansas
Udayan Apte, University of Kansas
Kathryn M. Taylor, Cardiff University, UK
Christian T. Sheline, LSU Health Sciences Center - New Orleans
Li Wang, University of Utah
Glen K. Andrews, University of Kansas

Document Type

Dataset

Publication Date

2-20-2013

Abstract

Part A: Frozen sections of HCC from patients 3, 4 and 11 and normal liver tissue from patient 3 (see Table 1) were fixed, permeablized and blocked then ZIP4 was detected using an antipeptide antibody against mouse ZIP4 [26], [47]. The peptide immunogen is well conserved between mouse and human and specificity of this antibody has been established [26], [27], [46]. Sections were washed and incubated sequentially with biotinylated secondary antibody then QDot 655 streptavidin conjugate. Red indicates the presence of ZIP4 whereas blue indicates nuclei stained with DAPI. Adobe Photoshop image capture software was used to capture these images. Part B: Frozen serial sections from patients 3 & 4 stained with hematoxylin and eosin to reveal the cellular structure of the ZIP4 positive tumors. Arrows point to the boundary of well-circumscribed lesions containing ZIP4 positive hepatocytes.

Comments

Original Article: https://doi.org/10.1371/journal.pone.0013158

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

Weaver, Benjamin P.; Zhang, Yuxia; Hiscox, Stephen; Guo, Grace L.; Apte, Udayan; Taylor, Kathryn M.; Sheline, Christian T.; Wang, Li; and Andrews, Glen K., "Datasets: Increased Expression of Zip4 (Slc39a4) May also Occur in Many Different Cancers" (2013). Data Sets. 105.
https://digitalscholar.lsuhsc.edu/datasets/105