CHARACTERIZATION OF HUMAN AMNIOTIC FLUID STEM CELLS (hAFSCs)
Publication Date
July 2019
Location
LSU Health Medical Education Building
Document Type
Abstract
Start Date
26-7-2019 9:00 AM
End Date
26-7-2019 12:00 PM
Description
Background: Amniotic fluid (AF) is an inexpensive and easily obtainable source of adult stem cells collected during third trimester elected cesarean sections. Human amniotic fluid stem cells (hAFSCs) are now considered a new source for therapy because of their ability to differentiated into multiple cell lineages. Aim: The aim of the present study is to characterize stem cells isolated from hAFSCs. Methods: Flow cytometry was performed on AF and hAFSCs. Colony forming unit (CFU) for day 14 and proliferation assays for day 1, 7, 14 and 21 were done for hAFSCs. Results: Flow cytometry data show that AF consists of a heterogeneous cell population of hAFSC that express the mesenchymal stem markers (CD90, CD73, and CD105), epithelial cells (CD326) and immune cell makers (CD14, CD3, CD45, CD117, MAC1). CFU data indicated the self-renew ability of hAFSCs. Proliferation data shows the exponential cell growth over a period of 21 days. Conclusion: The AF provides a novel source of stem cells with potential use in cellular therapy and regenerative medicine.
Recommended Citation
Byerly, Ann M., "CHARACTERIZATION OF HUMAN AMNIOTIC FLUID STEM CELLS (hAFSCs)" (2019). Summer Research Internship Program. 5.
https://digitalscholar.lsuhsc.edu/srip/2019/undergrad/5
CHARACTERIZATION OF HUMAN AMNIOTIC FLUID STEM CELLS (hAFSCs)
LSU Health Medical Education Building
Background: Amniotic fluid (AF) is an inexpensive and easily obtainable source of adult stem cells collected during third trimester elected cesarean sections. Human amniotic fluid stem cells (hAFSCs) are now considered a new source for therapy because of their ability to differentiated into multiple cell lineages. Aim: The aim of the present study is to characterize stem cells isolated from hAFSCs. Methods: Flow cytometry was performed on AF and hAFSCs. Colony forming unit (CFU) for day 14 and proliferation assays for day 1, 7, 14 and 21 were done for hAFSCs. Results: Flow cytometry data show that AF consists of a heterogeneous cell population of hAFSC that express the mesenchymal stem markers (CD90, CD73, and CD105), epithelial cells (CD326) and immune cell makers (CD14, CD3, CD45, CD117, MAC1). CFU data indicated the self-renew ability of hAFSCs. Proliferation data shows the exponential cell growth over a period of 21 days. Conclusion: The AF provides a novel source of stem cells with potential use in cellular therapy and regenerative medicine.
Comments
Mentors: Bruce Bunelle and Frank Lau, Center for Stem Cell Research and Regenerative Medicine, Tulane University & LSUHSC Surgery Department