Single-cell multiome landscape reveals elovanoid-mediated suppression of inflammatory glial states and induction of homeostatic signaling after ischemic stroke
Document Type
Article
Publication Date
8-29-2026
Publication Title
Experimental Neurology
Abstract
Ischemic stroke is a leading cause of death and disability. Administration of the lipid mediators elovanoids (ELVs) is protective in human neuronal-glia cultures and in experimental ischemic stroke. We now report using a single-cell multiome approach that intranasally-delivered (IN) ELV34 or its precursor reduced the loss of neuronal markers and upregulated homeostatic microglia signatures after stroke. Thus, ELV reduces disease-associated microglia (expressing Spp1, Gpnmb, Lgals3, Clec7a) and the expression of neuroinflammatory signaling genes.
In astrocytes, ELV decreased reactive astrocytes (expressing Gfap, Vim, Nes, Lcn2) and upregulated genes involved in synaptic organization. Also, ELV reduced abundance of oligodendrocytes and OPCs expressing immune markers. ELV induced a phenotype shift from pro-inflammatory microglia, astrocytes, oligodendrocytes, and OPCs in response to ischemic stroke damage. ELV upregulated gene pathways promoting synaptic integrity, reducing immune cell activation and neuronal loss.
First Page
1
Last Page
13
PubMed ID
42668080
Volume
406
Issue
115998
Publisher
Elsevier Inc.
Rights
© 2026 The Authors
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Recommended Citation
Ji, Jeff X.; Bhattacharjee, Surjyadipta; Giles, Brian L.; Belayev, Ludmila; and Bazan, Nicolas G., "Single-cell multiome landscape reveals elovanoid-mediated suppression of inflammatory glial states and induction of homeostatic signaling after ischemic stroke" (2026). School of Medicine Faculty Publications. 5219.
https://digitalscholar.lsuhsc.edu/som_facpubs/5219
10.1016/j.expneurol.2026.115998