Targeted inhibition of 7SK long non-coding RNA using Rn7SK-siRNA promotes gastric cancer cell proliferation and migration

Document Type

Article

Publication Date

10-3-2025

Publication Title

Gene Reports

Abstract

Background: With a length of 331 nucleotides, Rn7SK small nuclear RNA (snRNA) is an extremely plentiful noncoding RNA (ncRNA). Multiple lines of evidence propose that Rn7SK snRNA controls RNA polymerase II transcription by regulating the positive transcription elongation factor b (P-TEFb) function. Previous researches has shown that Rn7SK is downregulated in some tumor cells. This investigation aimed to analyze the impact of the downregulation of Rn7SK expression via transfection of Rn7SK-siRNA on proliferation, migration, and spheroid formation in human gastric adenocarcinoma cells AGS. Also, the expression level of genes engaged in cancer cell proliferation and apoptosis was assessed. Results: The obtained findings demonstrated that transfection of AGS cells with Rn7SK-siRNA increased their migration, proliferation, and spheroid formation. Besides, qPCR analysis revealed higher and lower expression levels of genes involved in proliferation and apoptosis, respectively, in Rn7SK-siRNA-transfected AGS cells. Conclusions: Our result suggests that Rn7SK has a crucial role in the progression of human gastric cancer AGS cells, and applying effective strategies to overexpress Rn7SK might help with the efficient treatment of gastric cancer.

Volume

41

Rights

© 2025 Elsevier Inc.

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