Document Type
Article
Publication Date
9-23-2024
Publication Title
Gastro Hep Advances
Abstract
Background and Aims: Pancreatic stellate cells (PSCs) are critical mediators in chronic pancreatitis with an undefined role in acute pancreatitis (AP). PSCs consist of a heterogenous group of cells and are considered interchangeable with pancreatic fibroblasts. This study explored the heterogeneous nature of PSCs by characterizing pancreatic collagen-expressing fibroblasts (PCFs) via lineage tracing in mouse normal and AP pancreas and determining the effect of PCF depletion in AP. Methods: Tandem dimer Tomato (tdTom+) PCFs in collagen type 1 (Col1)a2CreERtdTomato (Tom) mice receiving tamoxifen were characterized via fluorescence, Oil Red staining, and flow cytometry. AP was induced by cerulein, AP injury was assessed, and tdTom+ PCFs were monitored. The effect of PCF depletion on AP injury was evaluated in Col1a2CreERdiphtheria toxin A mice. Results: Approximately 13% of pancreatic cells in Col1a2CreERTom mice were labeled by tdTom (tdTom+ PCFs), which surrounded acini, ducts, and blood vessels, and stained with Oil Red, collagen type I, vimentin, and desmin. tdTom+ PCFs increased 2-fold during AP, correlating with AP score, amylase, and alpha-smooth muscle actin+ and Ki67+ staining. PCF depletion in Col1a2CreERdiphtheria toxin A mice receiving tamoxifen resulted in enhanced inflammation compared to control. Conclusion: PCFs may constitute a subset of PSCs and can be activated during AP. PCF depletion aggravates AP, suggesting a protective role for PCFs.
PubMed ID
39866719
Volume
4
Issue
2
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 International License.
Recommended Citation
Qin, Amy; Shi, Kevin; Tindall, Rachel R.; Li, Jiajing; Cheng, Binglu; Li, Jing; Yang, Baibing; Yu, Qiang; Zhang, Yinjie; Hong, Bangxing; Kaur, Balveen; Younes, Mamoun; Shen, Qiang; Bailey-Lundberg, Jennifer M.; Cao, Yanna; and Ko, Tien C., "Characterization of Pancreatic Collagen-Expressing Fibroblasts in Mouse Acute Pancreatitis" (2024). School of Medicine Faculty Publications. 3374.
https://digitalscholar.lsuhsc.edu/som_facpubs/3374
10.1016/j.gastha.2024.09.012
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