Relevance of lymphocyte proliferation to PHA in severe combined immunodeficiency (SCID) and T cell lymphopenia
Document Type
Article
Publication Date
2-15-2024
Publication Title
Clinical Immunology
Abstract
Severe combined immunodeficiency (SCID) is characterized by a severe deficiency in T cell numbers. We analyzed data collected (n = 307) for PHA-based T cell proliferation from the PIDTC SCID protocol 6901, using either a radioactive or flow cytometry method. In comparing the two groups, a smaller number of the patients tested by flow cytometry had < 10% of the lower limit of normal proliferation as compared to the radioactive method (p = 0.02). Further, in patients with CD3+ T cell counts between 51 and 300 cells/μL, there was a higher proliferative response with the PHA flow assay compared to the 3H-T assay (p < 0.0001), suggesting that the method of analysis influences the resolution and interpretation of PHA results. Importantly, we observed many SCID patients with profound T cell lymphopenia having normal T cell proliferation when assessed by flow cytometry. We recommend this test be considered only as supportive in the diagnosis of typical SCID.
PubMed ID
38367737
Volume
261
Recommended Citation
Abraham, Roshini S.; Basu, Amrita; Heimall, Jennifer R.; Dunn, Elizabeth; Yip, Alison; Kapadia, Malika; Kapoor, Neena; Satter, Lisa Forbes; Buckley, Rebecca; O'Reilly, Richard; Cuvelier, Geoffrey D.E.; Chandra, Sharat; Bednarski, Jeffrey; Chaudhury, Sonali; Moore, Theodore B.; Haines, Hilary; Dávila Saldaña, Blachy J.; Chellapandian, Deepakbabu; Rayes, Ahmad; Chen, Karin; Caywood, Emi; Chandrakasan, Shanmuganathan; Lugt, Mark Thomas Vander; Ebens, Christen; Teira, Pierre; Shereck, Evan; Miller, Holly; Aquino, Victor; and Yu, Lolie C., "Relevance of lymphocyte proliferation to PHA in severe combined immunodeficiency (SCID) and T cell lymphopenia" (2024). School of Medicine Faculty Publications. 2450.
https://digitalscholar.lsuhsc.edu/som_facpubs/2450