In Vitro Culture of White Adipose Tissue
Document Type
Book Chapter
Publication Date
3-14-2024
Publication Title
Methods in Molecular Biology
Abstract
White adipose tissue (WAT) plays a crucial endocrine organ that regulates blood glucose and lipid levels, satiety, and inflammation. Before the described technique, primary white adipocytes could not be stably cultured in vitro. The lack of a reliable primary culture model impeded research in WAT metabolism and drug development. We have developed a novel technique for WAT primary culture called "sandwiched white adipose tissue" (SWAT). SWAT overcomes the natural buoyancy of adipocytes by sandwiching minced WAT between sheets of adipose-derived stromal cells. The resulting constructs are viable for at least 8 weeks in culture. SWAT maintains the intact extracellular matrix, cell-to-cell contacts, and physical pressures of in vivo WAT conditions; additionally, SWAT maintains a robust transcriptional profile, sensitivity to exogenous chemical signaling, and whole tissue function. SWAT represents a simple, reproducible, and effective method of primary adipose culture. Potentially, it is a broadly applicable platform for research in WAT physiology, pathophysiology, metabolism, and pharmaceutical development.
First Page
279
Last Page
285
PubMed ID
38478240
Volume
2783
Recommended Citation
Fontenot, Jake J. and Lau, Frank H., "In Vitro Culture of White Adipose Tissue" (2024). School of Medicine Faculty Publications. 2433.
https://digitalscholar.lsuhsc.edu/som_facpubs/2433
10.1007/978-1-0716-3762-3_19