Document Type
Article
Publication Date
1-16-2024
Publication Title
Journal of Biological Engineering
Abstract
Gene delivery to, and expression in, the mouse brain is important for understanding gene functions in brain development and disease, or testing gene therapies. Here, we describe an approach to express a transgene in the mouse brain in a cell-type-specific manner. We use stereotaxic injection of a transgene-expressing adeno-associated virus into the mouse brain via the intracerebroventricular route. We demonstrate stable and sustained expression of the transgene in neurons of adult mouse brain, using a reporter gene driven by a neuron-specific promoter. This approach represents a rapid, simple, and cost-effective method for global gene expression in the mouse brain, in a cell-type-specific manner, without major surgical interventions. The described method represents a helpful resource for genetically engineering mice to express a therapeutic gene, for gene therapy studies, or to deliver genetic material for genome editing and developing knockout animal models.
PubMed ID
38229168
Volume
18
Issue
1
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Ahmed, Osama; Ekumi, Kingsley M.; Nardi, Francesco V.; Maisumu, Gulimiheranmu; Moussawi, Khaled; Lazartigues, Eric D.; Liang, Bo; and Yakoub, Abraam M., "Stable, neuron-specific gene expression in the mouse brain" (2024). School of Medicine Faculty Publications. 2093.
https://digitalscholar.lsuhsc.edu/som_facpubs/2093
10.1186/s13036-023-00400-5