Relative and Quantitative Characterization of the Bovine Bacterial Ocular Surface Microbiome in the Context of Suspected Ocular Squamous Cell Carcinoma

Authors

Hannah B. Gafen, Louisiana State University, Baton Rouge, LA
Chin-Chi Liu, Louisiana State University, Baton Rouge, LA
Nikole E. Ineck, Louisiana State University, Baton Rouge, LA
Clare M. Scully, Louisiana State University, Baton Rouge, LA
Melanie A. Mironovich, Louisiana State University, Baton Rouge, LA
Lauren Guarneri, Louisiana State University, Baton Rouge, LA
Christopher M. Taylor, LSU Health Sciences Center - New OrleansFollow
Meng Luo, LSU Health Sciences Center - New OrleansFollow
Marina L. Leis, Western College of Veterinary Medicine, Saskatoon, SK
Erin M. Scott, Cornell University, Ithaca, NY
Renee T. Carter, Louisiana State University, Baton Rouge, LA
Andrew C. Lewin, Louisiana State University, Baton Rouge, LA

Document Type

Article

Publication Date

6-13-2023

Publication Title

Animals

Abstract

The ocular surface microbiome is altered in certain disease states. The aim of this study was to characterize the bovine bacterial ocular surface microbiome (BBOSM) in the context of ocular squamous cell carcinoma (OSCC). The conjunctiva of normal (n = 28) and OSCC (n = 10) eyes of cows aged 2 to 13 years from two farms in Louisiana and Wyoming were sampled using individual sterile swabs. DNA extraction followed by 16S ribosomal ribonucleic acid (rRNA) gene sequencing and real-time polymerase chain reaction (RT-PCR) were performed to, respectively, assess the relative and absolute BBOSM. Discriminant analysis (DA) was performed using RT-PCR data, and relative abundance analysis was performed using 16S rRNA gene sequencing data. The 11 most abundant phyla in both normal and OSCC-affected cows were identified using 16S rRNA gene sequencing analysis. The relative abundance of Euryarchaeota was found to be significantly lower (p = 0.0372) in OSCC eyes compared to normal eyes. Relative abundance differences within and between geographic locations were also identified. Quadratic DA categorized samples as OSCC or normal with 100% sensitivity and 83.3–100% specificity. Relative abundance analysis identified relative BBOSM phylum alterations in OSCC. Quadratic DA can be used to accurately categorize BBOSM from normal and OSCC ocular surface samples.

PubMed ID

37370486

Volume

13

Issue

12

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.

Recommended Citation

Gafen, Hannah B.; Liu, Chin-Chi; Ineck, Nikole E.; Scully, Clare M.; Mironovich, Melanie A.; Guarneri, Lauren; Taylor, Christopher M.; Luo, Meng; Leis, Marina L.; Scott, Erin M.; Carter, Renee T.; and Lewin, Andrew C., "Relative and Quantitative Characterization of the Bovine Bacterial Ocular Surface Microbiome in the Context of Suspected Ocular Squamous Cell Carcinoma" (2023). School of Medicine Faculty Publications. 2055.
https://digitalscholar.lsuhsc.edu/som_facpubs/2055
10.3390/ani13121976