Isolation and culture of rat embryonic neural cells: a quick protocol
Journal of Visualized Experiments : JoVE
We are describing a quick method to dissociate and culture hippocampal or cortical neurons from E15-17 rat embryos. The procedure can be applied successfully to the isolation of mouse and human primary neurons and neural progenitors. Dissociated neurons are maintained in serum-free medium up to several weeks. These cultures can be used for nucleofection, immunocytochemistry, nucleic acids preparation, as well as electrophysiology. Older neuronal cultures can also be transfected with a good efficiency rate by lentiviral transduction and, less efficiently, with calcium phosphate or lipid-based methods such as lipofectamine.
Pacifici, Marco and Peruzzi, Francesca, "Isolation and culture of rat embryonic neural cells: a quick protocol" (2012). School of Medicine Faculty Publications. 2000.