Isolation and culture of rat embryonic neural cells: a quick protocol

Document Type

Article

Publication Date

5-24-2012

Publication Title

Journal of Visualized Experiments : JoVE

Abstract

We are describing a quick method to dissociate and culture hippocampal or cortical neurons from E15-17 rat embryos. The procedure can be applied successfully to the isolation of mouse and human primary neurons and neural progenitors. Dissociated neurons are maintained in serum-free medium up to several weeks. These cultures can be used for nucleofection, immunocytochemistry, nucleic acids preparation, as well as electrophysiology. Older neuronal cultures can also be transfected with a good efficiency rate by lentiviral transduction and, less efficiently, with calcium phosphate or lipid-based methods such as lipofectamine.

First Page

e3965

PubMed ID

22664838

Issue

63

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