Document Type
Article
Publication Date
9-13-2023
Publication Title
Cells
Abstract
Preclinical studies have shown that chronic alcohol abuse leads to alterations in the gastrointestinal microbiota that are associated with behavior changes, physiological alterations, and immunological effects. However, such studies have been limited in their ability to evaluate the direct effects of alcohol-associated dysbiosis. To address this, we developed a humanized alcohol-microbiota mouse model to systematically evaluate the immunological effects of chronic alcohol abuse mediated by intestinal dysbiosis. Germ-free mice were colonized with human fecal microbiota from individuals with high and low Alcohol Use Disorders Identification Test (AUDIT) scores and bred to produce human alcohol-associated microbiota or human control-microbiota F1 progenies. F1 offspring colonized with fecal microbiota from individuals with high AUDIT scores had increased susceptibility to Klebsiella pneumoniae and Streptococcus pneumoniae pneumonia, as determined by increased mortality rates, pulmonary bacterial burden, and post-infection lung damage. These findings highlight the importance of considering both the direct effects of alcohol and alcohol-induced dysbiosis when investigating the mechanisms behind alcohol-related disorders and treatment strategies.
PubMed ID
37759490
Volume
12
Issue
18
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Cunningham, Kelly C.; Smith, Deandra R.; Villageliú, Daniel N.; Ellis, Christi M.; Ramer-Tait, Amanda E.; Price, Jeffrey D.; Wyatt, Todd A.; Knoell, Daren L.; Samuelson, Mystera M.; Molina, Patricia E.; Welsh, David A.; and Samuelson, Derrick R., "Human Alcohol-Microbiota Mice have Increased Susceptibility to Bacterial Pneumonia" (2023). School of Medicine Faculty Publications. 1560.
https://digitalscholar.lsuhsc.edu/som_facpubs/1560
10.3390/cells12182267
Included in
Bacterial Infections and Mycoses Commons, Biological Phenomena, Cell Phenomena, and Immunity Commons, Medical Physiology Commons, Substance Abuse and Addiction Commons