Introduction To Immunohistochemistry: From To Evolving Science To Timeless Art

Document Type

Book Chapter

Publication Date

12-3-2021

Publication Title

Methods in Molecular Biology

Abstract

Immunohistochemistry and all techniques that use antibodies and fluorescence are widespread, essential and irreplaceable tools used in both research laboratory settings and diagnostic pathology laboratories. The field was born approximately 80 years ago, with the idea that antibodies could be tagged with fluorescent substances and used to detect antigens in cells and microorganisms, and has vertiginously evolved since; these advances have come in all aspects of the methodology, tissue fixation, generation of antibodies, monoclonal antibodies, signal amplification, antigen retrieval, signal amplification, microscopy and have become increasingly sophisticated, from in situ hybridization, in situ proximity ligation assay, flow cytometry, comet assay, to multiplexing and green fluorescent protein reconstitution, yielding Nobel Prizes along the way and generating invaluable scientific and diagnostic advances as well as timeless beautiful images.

First Page

1

Last Page

16

PubMed ID

34859395

Volume

2422

Comments

Immunohistochemistry and Immunocytochemistry Methods and Protocols

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