Er+ Breast Cancer Mammosphere Formation And Analysis
Methods in Molecular Biology
Mammosphere formation assays are a popular and convenient technique in the study of breast cancer by providing an in vitro mechanism by which to study breast cancer stem cell (BCSC) contribution to tumorigenesis, as well as more closely mimicking the three-dimensional tumor microenvironment. In these assays, BCSCs are stimulated to proliferate in low adherence tissue culture dishes and the resulting mammospheres exhibit activation of stem cell-related signaling pathways. Here we describe the process for generating and analyzing mammospheres under varying conditions.
Singleton, Ciera S.; Chan, Leo Li Ying; McCulley, Kelsey J.; Kessel, Sarah L.; Del Valle, Luis; and Crabtree, Judy S., "Er+ Breast Cancer Mammosphere Formation And Analysis" (2021). School of Medicine Faculty Publications. 1503.