Immunohistochemistry and Immunocytochemistry | Chapter 16
Document Type
Book Chapter
Publication Date
12-3-2021
Editor
Luis D. Valle
Abstract
Mammosphere formation assays are a popular and convenient technique in the study of breast cancer by providing an in vitro mechanism by which to study breast cancer stem cell (BCSC) contribution to tumorigenesis, as well as more closely mimicking the three-dimensional tumor microenvironment. In these assays, BCSCs are stimulated to proliferate in low adherence tissue culture dishes and the resulting mammospheres exhibit activation of stem cell-related signaling pathways. Here we describe the process for generating and analyzing mammospheres under varying conditions.
First Page
233
Last Page
245
PubMed ID
34859410
Volume
2422
Chapter Title
Chapter 16 - Er+ Breast Cancer Mammosphere Formation And Analysis
Recommended Citation
Singleton, Ciera S.; Chan, Leo Li Ying; McCulley, Kelsey J.; Kessel, Sarah L.; Del Valle, Luis; and Crabtree, Judy S., "Immunohistochemistry and Immunocytochemistry | Chapter 16" (2021). School of Medicine Faculty Publications. 1503.
https://digitalscholar.lsuhsc.edu/som_facpubs/1503
10.1007/978-1-0716-1948-3_16
Comments
Immunohistochemistry and Immunocytochemistry Methods and Protocols