Er+ Breast Cancer Mammosphere Formation And Analysis

Authors

Ciera S. Singleton, LSU Health Sciences Center - New Orleans
Leo Li Ying Chan, Nexcelom Bioscience LLC
Kelsey J. McCulley, Nexcelom Bioscience LLC
Sarah L. Kessel, Nexcelom Bioscience LLC
Luis Del Valle, LSU Health Sciences Center - New OrleansFollow
Judy S. Crabtree, LSU Health Sciences Center - New OrleansFollow

Document Type

Book Chapter

Publication Date

12-3-2021

Publication Title

Methods in Molecular Biology

Abstract

Mammosphere formation assays are a popular and convenient technique in the study of breast cancer by providing an in vitro mechanism by which to study breast cancer stem cell (BCSC) contribution to tumorigenesis, as well as more closely mimicking the three-dimensional tumor microenvironment. In these assays, BCSCs are stimulated to proliferate in low adherence tissue culture dishes and the resulting mammospheres exhibit activation of stem cell-related signaling pathways. Here we describe the process for generating and analyzing mammospheres under varying conditions.

First Page

233

Last Page

245

PubMed ID

34859410

Volume

2422

Comments

Immunohistochemistry and Immunocytochemistry Methods and Protocols

Recommended Citation

Singleton, Ciera S.; Chan, Leo Li Ying; McCulley, Kelsey J.; Kessel, Sarah L.; Del Valle, Luis; and Crabtree, Judy S., "Er+ Breast Cancer Mammosphere Formation And Analysis" (2021). School of Medicine Faculty Publications. 1503.
https://digitalscholar.lsuhsc.edu/som_facpubs/1503