Light-induced OsLIKE1 phosphorylation enhances rice resistance against blast disease

Authors

Jiangtao Liu, Nanjing Agricultural University, Nanjin, China
Lei Su, Nanjing Agricultural University, Nanjin, China
Wenya Wang, Nanjing Agricultural University, Nanjin, China
Jintao Wang, Nanjing Agricultural University, Nanjin, China
Weizhong Chen, Nanjing Agricultural University, Nanjin, China
Zhixiang Yang, Nanjing Agricultural University, Nanjin, China
Leiyun Yang, Nanjing Agricultural University, Nanjin, China
Gang Li, Nanjing Agricultural University, Nanjin, China
Xinyu Liu, Nanjing Agricultural University, Nanjin, China
Haifeng Zhang, Nanjing Agricultural University, Nanjin, China
Zhongqiang Qi, Jiangsu Academy of Agricultural Science, Nanjing, China
Yongfeng Liu, Jiangsu Academy of Agricultural Science, Nanjing, China
Yuqiang Liu, Nanjing Agricultural University, Nanjing, China
Ping Wang, LSU Health Sciences Center - New OrleansFollow
Muxing Liu, Nanjing Agricultural University, Nanjing, China.
Zhengguang Zhang, Nanjing Agricultural University, Nanjing, China.

Document Type

Article

Publication Date

12-23-2025

Publication Title

Nature Communications

Abstract

Rice blast caused by Magnaporthe oryzae poses a major threat to world food supply. Conditions conducive to the disease include insufficient light. Our previous studies found that light-dependent phosphorylation of light-harvesting complex II protein (LHCB5) mediates rice immunity; however, the underlying mechanisms, in particular, the upstream regulators of LHCB5 phosphorylation, remain unknown. Here, we show that OsLIKE1 functions as a lectin receptor-like kinase (LecRLK) to play a critical role in rice resistance against M. oryzae. OsLIKE1 interacts with and phosphorylates LHCB5. In addition, we find two phosphorylation sites in the kinase domain of OsLIKE1 important for its auto-phosphorylation and phosphorylation of LHCB5. Moreover, we find that variation in light-induced OsLIKE1 expression is driven by specific single-nucleotide polymorphisms (SNPs) within the promoter region. These SNPs promote the auto-phosphorylation of OsLIKE1 to phosphorylate LHCB5 and activate its immune function against the blast fungus. Collectively, these findings elucidate the mechanism by which light regulates OsLIKE1-dependent defense in rice and support SNP-based rice breeding programs that increase disease resistance.

PubMed ID

41436453

Volume

17

Issue

1

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.

Recommended Citation

Liu, Jiangtao; Su, Lei; Wang, Wenya; Wang, Jintao; Chen, Weizhong; Yang, Zhixiang; Yang, Leiyun; Li, Gang; Liu, Xinyu; Zhang, Haifeng; Qi, Zhongqiang; Liu, Yongfeng; Liu, Yuqiang; Wang, Ping; Liu, Muxing; and Zhang, Zhengguang, "Light-induced OsLIKE1 phosphorylation enhances rice resistance against blast disease" (2025). School of Graduate Studies Faculty Publications. 466.
https://digitalscholar.lsuhsc.edu/sogs_facpubs/466
10.1038/s41467-025-67504-y