A Repetitive Acipenser gueldenstaedtii Genomic Region Aligning with the Acipenser baerii IGLV Gene Cluster Suggests a Role as a Transcription Termination Element Across Several Sturgeon Species

Authors

• Alexander V. Chouljenko, North Carolina State University, Morehead City, NC
• Brent A. Stanfield, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA
• Tetiana O. Melnyk, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA
• Ojasvi Dutta, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA
• Vladimir N. Chouljenko, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA

Document Type

Article

Publication Date

11-26-2024

Publication Title

International Journal of Molecular Sciences

Abstract

This study focuses on the common presence of repetitive sequences within the sturgeon genome that may contribute to enhanced immune responses against infectious diseases. A repetitive 675 bp VAC-2M sequence in Russian sturgeon DNA that aligns with the Siberian sturgeon IGLV gene cluster was identified. A specific 218 bp long portion of the sequence was found to be identical between Acipenser gueldenstaedtii, A. baerii and A. stellatus species, and NCBI blast analysis confirmed the presence of this DNA segment in the A. ruthenus genome. Multiple mutated copies of the same genomic region were detected by PCR analysis, indicating that different versions of this highly repetitive sequence exist simultaneously within the same organism. The selection toward specific genetic differences appears to be highly conserved based on the sequence variations within DNA originating from fish grown at distant geographical regions and individual caviar grains from the same fish. The corresponding A. baerii genomic region encompassing the 357 bp DNA sequence was cloned either ahead or after the human cytomegalovirus immediate early promoter (HCMV-IE) into a pBV-Luc reporter vector expressing the luciferase gene. The DNA segment significantly reduced luciferase expression in transient transfection/expression experiments. The results indicate that this genomic region functions as a transcription termination element that may affect antibody production in sturgeons.

First Page

1*

Last Page

18

DOI

10.3390/ijms252312685

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

Chouljenko, Alexander V.; Stanfield, Brent A.; Melnyk, Tetiana O.; Dutta, Ojasvi; and Chouljenko, Vladimir N., "A Repetitive Acipenser gueldenstaedtii Genomic Region Aligning with the Acipenser baerii IGLV Gene Cluster Suggests a Role as a Transcription Termination Element Across Several Sturgeon Species" (2024). LSU-LCMC Cancer Center Faculty Publications. 228.
https://digitalscholar.lsuhsc.edu/llcc_facpubs/228
10.3390/ijms252312685