Replication kinetics and cytopathic effect of feline calicivirus in feline corneal epithelial cells

Authors

• Daniela I. Saade, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA
• Chin-Chi Liu, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA
• Erinn P. Mills, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA
• Brent Stanfield, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA
• Côme J. Thieulent, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA
• Vladimir N. Chouljenko, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA
• Ugochi Emelogu, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA
• Renee T. Carter, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA
• Pilar Camacho-Luna, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA
• Andrew C. Lewin, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA

Document Type

Article

Publication Date

10-8-2024

Publication Title

Veterinary Opthalmology

Abstract

Objective To determine the replication kinetics and cytopathic effect (CPE) of feline calicivirus (FCV) in feline corneal epithelial cells (FCEC). Animals Studied Seven archived FCV isolates and one archived feline herpesvirus type 1 (FHV-1) isolate, previously obtained from eight domestic short hair cats. Procedures FCV RNA was extracted for sequencing using Illumina MiSeq, to identify three genomically diverse isolates for further testing. Following reference-based assembly, viral genomes were annotated and assessed. Superficial keratectomies were performed to isolate the corneal epithelium of cats and the cells were cultured in vitro. FCEC were infected with the three chosen FCV isolates and one FHV-1 isolate at two different multiplicity of infection ratios (MOIs, 0.1 and 0.01 PFU/cell) and virus titration was assessed at 0, 2, 6, 12, 24, and 48 h post-infection (hpi). Viral identity was confirmed by RT-qPCR. Results Three genomically diverse FCV isolates were chosen for further assessment in the FCEC model. All infections of FCEC with FCV led to visible CPE, characterized by epithelial cell rounding and detachment from the plate by 24 hpi, while FHV-1 led to visible CPE within 48 hpi. All three of the FCV isolates replicated effectively in FCEC at both 0.1 and 0.01 MOI, with a peak increase in titer approximately 12–24 hpi. Conclusions The results support the possible role of FCV as a primary pathogen of the feline ocular surface. FCV replicates in FCEC in vitro, leading to profound CPE.

First Page

1*

Last Page

11

DOI

10.1111/vop.13289

Rights

© 2024 American College of Veterinary Ophthalmologists.

Recommended Citation

Saade, Daniela I.; Liu, Chin-Chi; Mills, Erinn P.; Stanfield, Brent; Thieulent, Côme J.; Chouljenko, Vladimir N.; Emelogu, Ugochi; Carter, Renee T.; Camacho-Luna, Pilar; and Lewin, Andrew C., "Replication kinetics and cytopathic effect of feline calicivirus in feline corneal epithelial cells" (2024). LSU-LCMC Cancer Center Faculty Publications. 117.
https://digitalscholar.lsuhsc.edu/llcc_facpubs/117
10.1111/vop.13289