DETECTION OF EPSTEIN-BARR VIRUS AND HUMAN PAPALLOMAVIRUS IN RESIDUAL PAP SMEAR FLUIDS IN HUMAN IMMUNODEFICIENCY VIRUS+ WOMEN
Document Type
Presentation
Start Date
22-10-2010 3:00 PM
End Date
22-10-2010 4:30 PM
Description
Human Papillomavirus (HPV) is the most common viral sexually transmitted infection and select types, such as HPV 16 and 18, are considered cancer-causing. A small fraction of infected women develop cervical dysplasia or cancer despite the high prevalence rate of HPV infections. HPV is required for the development of cervical cancer, however, it is not sufficient alone. This indicates the possible role of a cofactor, like the oncogenic Epstein-Barr virus (EBV). Preliminary studies demonstrated co-detection of EBV with High-Risk HPV did increase the risk of cervical dysplasia in HIV+ women. It is not known if EBV can also be detected in residual Pap smear fluid which could improve this clinical test.
Residual Pap smear fluid was obtained from 118 HIV+ female participants at the HIV Outpatient Program (HOP). DNA extractions were performed using a Qiagen QiAamp DNA blood mini Kit. PCR was used to detect human DNA (beta-globin) and determine the prevalence of EBV and HPV. HPV genotyping was performed with the reverse line blot assay. The presence of EBV was correlated with HPV results and concurrent Pap smear findings.
The subjects were predominately single African American women, with a median age of 45 years, average HIV viral load of 28,692 and average CD4 count of 485. EBV and HPV were detected in, 50% and 15.6%, respectively, of the Pap smear specimens. Co-shedding was present in 8.3% of the samples. Further analysis indicates that EBV co-shedding was present in 75% of women with an abnormal Pap smear. However, in this small study, there was no association of co-detection with an abnormal Pap smear (p= 0.88).
EBV can be readily detected in residual Pap smear fluid from HIV+ individuals. High rates of abnormal pap smears are found in women co-shedding HPV and EBV. This underscores the potential to utilize EBV with HPV testing as an improved marker for abnormal Pap smears.
Recommended Citation
Nichols, Whitney; Nelson, N.; Clark, A.; and Oddo, H., "DETECTION OF EPSTEIN-BARR VIRUS AND HUMAN PAPALLOMAVIRUS IN RESIDUAL PAP SMEAR FLUIDS IN HUMAN IMMUNODEFICIENCY VIRUS+ WOMEN" (2010). Dr. Joseph M. Moerschbaecher, III Graduate Research Day. 14.
https://digitalscholar.lsuhsc.edu/grad_rs/2010/poster2/14
DETECTION OF EPSTEIN-BARR VIRUS AND HUMAN PAPALLOMAVIRUS IN RESIDUAL PAP SMEAR FLUIDS IN HUMAN IMMUNODEFICIENCY VIRUS+ WOMEN
Human Papillomavirus (HPV) is the most common viral sexually transmitted infection and select types, such as HPV 16 and 18, are considered cancer-causing. A small fraction of infected women develop cervical dysplasia or cancer despite the high prevalence rate of HPV infections. HPV is required for the development of cervical cancer, however, it is not sufficient alone. This indicates the possible role of a cofactor, like the oncogenic Epstein-Barr virus (EBV). Preliminary studies demonstrated co-detection of EBV with High-Risk HPV did increase the risk of cervical dysplasia in HIV+ women. It is not known if EBV can also be detected in residual Pap smear fluid which could improve this clinical test.
Residual Pap smear fluid was obtained from 118 HIV+ female participants at the HIV Outpatient Program (HOP). DNA extractions were performed using a Qiagen QiAamp DNA blood mini Kit. PCR was used to detect human DNA (beta-globin) and determine the prevalence of EBV and HPV. HPV genotyping was performed with the reverse line blot assay. The presence of EBV was correlated with HPV results and concurrent Pap smear findings.
The subjects were predominately single African American women, with a median age of 45 years, average HIV viral load of 28,692 and average CD4 count of 485. EBV and HPV were detected in, 50% and 15.6%, respectively, of the Pap smear specimens. Co-shedding was present in 8.3% of the samples. Further analysis indicates that EBV co-shedding was present in 75% of women with an abnormal Pap smear. However, in this small study, there was no association of co-detection with an abnormal Pap smear (p= 0.88).
EBV can be readily detected in residual Pap smear fluid from HIV+ individuals. High rates of abnormal pap smears are found in women co-shedding HPV and EBV. This underscores the potential to utilize EBV with HPV testing as an improved marker for abnormal Pap smears.
Comments
See abstract book page 58