COMPARISONS OF LESS-INVASIVE SAMPLING TECHNIQUES FOR HUMAN PAPILLOMA VIRUS
Document Type
Presentation
Start Date
22-10-2010 3:00 PM
End Date
22-10-2010 4:30 PM
Description
Human Papillomavirus (HPV) is the most common sexually transmitted disease and also causes cervical cancer. Recently, studies have shown that a single HPV DNA test is more predictable at determining cervical pathology than a single cervical pap smear. Currently, HPV testing is performed by an invasive cervical exam. Here we look at different less-invasive sampling techniques to see if they are concordant with the HPV results obtained from the cervical exam of our patients.
We obtained a cervical, urine, tampon, and/or self swab sample from 73 patients in an ongoing longitudinal HPV study of HIV+ women. The DNA was extracted from these samples using the Qiagen kit, HPV detected by PCR and HPV genotype determined by reverse line blot.
The medical provider obtained cervical sample was used as the gold standard and was compared to the other techniques. Of the 73 cervical samples tested 70 (95%) were positive for beta-globin and 60 (86%) were positive for HPV. The urine had 69 samples and 61 (88%) were positive for beta-globin and 24 (39%) were positive for HPV. There were 35 tampon samples where 32 (91 %) were beta-globin positive and 18 (57%) were HPV positive. The self swabs had 29 samples with 28 (97%) beta-globin positive and 21 (75%) HPV positive. We also compared the concordance of the detection of HPV between the cervical samples and the other samples. We found that 44% of the urine and cervical samples were concordant (p=0.60), whereas the self swabs and cervical samples were 82% concordant (p=0.009) and the tampons and cervical samples were 72% concordant (p=0.005).
Based on this data, HPV can be detected in the urine, tampon, and self swab samples. The tampons and self swabs are concordant with the cervical samples. Further studies are ongoing to validate these findings in larger cohorts of HIV+ and HIV-negative women.
Recommended Citation
Nelson, Nia; Suttom, K.; Nichols, W.; and Clark, A., "COMPARISONS OF LESS-INVASIVE SAMPLING TECHNIQUES FOR HUMAN PAPILLOMA VIRUS" (2010). Dr. Joseph M. Moerschbaecher, III Graduate Research Day. 13.
https://digitalscholar.lsuhsc.edu/grad_rs/2010/poster2/13
COMPARISONS OF LESS-INVASIVE SAMPLING TECHNIQUES FOR HUMAN PAPILLOMA VIRUS
Human Papillomavirus (HPV) is the most common sexually transmitted disease and also causes cervical cancer. Recently, studies have shown that a single HPV DNA test is more predictable at determining cervical pathology than a single cervical pap smear. Currently, HPV testing is performed by an invasive cervical exam. Here we look at different less-invasive sampling techniques to see if they are concordant with the HPV results obtained from the cervical exam of our patients.
We obtained a cervical, urine, tampon, and/or self swab sample from 73 patients in an ongoing longitudinal HPV study of HIV+ women. The DNA was extracted from these samples using the Qiagen kit, HPV detected by PCR and HPV genotype determined by reverse line blot.
The medical provider obtained cervical sample was used as the gold standard and was compared to the other techniques. Of the 73 cervical samples tested 70 (95%) were positive for beta-globin and 60 (86%) were positive for HPV. The urine had 69 samples and 61 (88%) were positive for beta-globin and 24 (39%) were positive for HPV. There were 35 tampon samples where 32 (91 %) were beta-globin positive and 18 (57%) were HPV positive. The self swabs had 29 samples with 28 (97%) beta-globin positive and 21 (75%) HPV positive. We also compared the concordance of the detection of HPV between the cervical samples and the other samples. We found that 44% of the urine and cervical samples were concordant (p=0.60), whereas the self swabs and cervical samples were 82% concordant (p=0.009) and the tampons and cervical samples were 72% concordant (p=0.005).
Based on this data, HPV can be detected in the urine, tampon, and self swab samples. The tampons and self swabs are concordant with the cervical samples. Further studies are ongoing to validate these findings in larger cohorts of HIV+ and HIV-negative women.
Comments
See abstract book page 57