14S,21 R-DIHYDROXY DOCOSAHEXAENOIC ACID PROMOTES WOUND HEALING
Document Type
Presentation
Start Date
22-10-2010 10:45 AM
End Date
22-10-2010 12:00 PM
Description
Purpose-Based on the fact DHA is relatively abundant in wounded skin, and can be converted into bioactive lipid mediators, we hypothesized that wounding could induce this biosynthesis to generate a new lipid mediator, 14S,21-dihydroxy-docosahexaenoic acid (14S,21 R-diHDHA), which promote wound healing.
Methods-Biosynthesis of 14S,21 R-diHDHA was determined by LC-.UV-MS/MS analysis. 14S,21 R-diHDHA accelerating wound healing was assessed in splinted-excisional-wound healing models in C57BL/6J mice, as well as by C57BL/6J dermal microvascular endothelial cells (DMVECs) migration, vasculature formation and vascular endothelial growth factor (VEGF) expression in vitro. Phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK) was valued by Western blot.
Results-Here we identified the formations of 14S,21 R-diHDHA in cutaneous wounds of C57BL/6J mice. Supplementation of exogenous 14S,21 R-diHDHA to wounds in animals rescued impaired healing and angiogenesis by accelerating wound re-epithelialization, granulation tissue formation, and vascularization. In the pivotal cellular processes of angiogenesis, 14S,21 RdiHDHA enhanced VEGF release, migration, and vasculature formation of DMVECs. Western blot revealed that 14S,21 R-diHDHA enhanceed phosphorylation of p38 MAPK in DMVECs.
Conclusion-Lipid mediator 14S,21 R-diHDHA promotes wound healing, and is a new lead for the development of better therapeutics treating impaired wound healing.
Recommended Citation
Tian, Haibin; Lu, Yan; Shah, Shraddha P.; and Hong, Song, "14S,21 R-DIHYDROXY DOCOSAHEXAENOIC ACID PROMOTES WOUND HEALING" (2010). Dr. Joseph M. Moerschbaecher, III Graduate Research Day. 22.
https://digitalscholar.lsuhsc.edu/grad_rs/2010/poster1/22
14S,21 R-DIHYDROXY DOCOSAHEXAENOIC ACID PROMOTES WOUND HEALING
Purpose-Based on the fact DHA is relatively abundant in wounded skin, and can be converted into bioactive lipid mediators, we hypothesized that wounding could induce this biosynthesis to generate a new lipid mediator, 14S,21-dihydroxy-docosahexaenoic acid (14S,21 R-diHDHA), which promote wound healing.
Methods-Biosynthesis of 14S,21 R-diHDHA was determined by LC-.UV-MS/MS analysis. 14S,21 R-diHDHA accelerating wound healing was assessed in splinted-excisional-wound healing models in C57BL/6J mice, as well as by C57BL/6J dermal microvascular endothelial cells (DMVECs) migration, vasculature formation and vascular endothelial growth factor (VEGF) expression in vitro. Phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK) was valued by Western blot.
Results-Here we identified the formations of 14S,21 R-diHDHA in cutaneous wounds of C57BL/6J mice. Supplementation of exogenous 14S,21 R-diHDHA to wounds in animals rescued impaired healing and angiogenesis by accelerating wound re-epithelialization, granulation tissue formation, and vascularization. In the pivotal cellular processes of angiogenesis, 14S,21 RdiHDHA enhanced VEGF release, migration, and vasculature formation of DMVECs. Western blot revealed that 14S,21 R-diHDHA enhanceed phosphorylation of p38 MAPK in DMVECs.
Conclusion-Lipid mediator 14S,21 R-diHDHA promotes wound healing, and is a new lead for the development of better therapeutics treating impaired wound healing.