Bioavailability and spatial distribution of fatty acids in the rat retina after dietary omega-3 supplementation
Journal of Lipid Research
Spatial changes of FAs in the retina in response to different dietary n-3 formulations have never been explored, although a diet rich in EPA and DHA is recommended to protect the retina against the effects of aging. In this study, Wistar rats were fed for 8 weeks with balanced diet including either EPA-containing phospholipids (PLs), EPA-containing TGs, DHA-containing PLs, or DHA-containing TGs. Qualitative changes in FA composition of plasma, erythrocytes, and retina were evaluated by gas chromatography-flame ionization detector. Following the different dietary intakes, changes to the quantity and spatial organization of PC and PE species in retina were determined by LC coupled to MS/ MS and MALDI coupled to MS imaging. The omega-3 content in the lipids of plasma and erythrocytes suggests that PLs as well as TGs are good omega-3 carriers for retina. However, a significant increase in DHA content in retina was observed, especially molecular species as di-DHA-containing PC and PE, as well as an increase in very long chain PUFAs (more than 28 carbons) following PL-EPA and TG-DHA diets only. All supplemented diets triggered spatial organization changes of DHA in the photoreceptor layer around the optic nerve. Taken together, these findings suggest that dietary omega-3 supplementation can modify the content of FAs in the rat retina.
Vidal, Elisa; Jun, Bokkyoo; Gordon, William C.; Maire, Marie Annick; Martine, Lucy; Grégoire, Stéphane; Khoury, Spiro; Cabaret, Stephanie; Berdeaux, Olivier; Acar, Niyazi; Bretillon, Lionel; and Bazan, Nicolas G., "Bioavailability and spatial distribution of fatty acids in the rat retina after dietary omega-3 supplementation" (2020). School of Medicine Faculty Publications. 1532.