Document Type
Article
Publication Date
9-6-2024
Publication Title
Antibody Therapeutics
Abstract
Active immunization (vaccination) induces long-lasting immunity with memory, which takes weeks to months to develop. Passive immunization (transfer of neutralizing antibodies) provides immediate protection, yet with high cost and effects being comparatively short-lived. No currently approved adjuvants are compatible with formulations to combine active and passive immunizations, not to mention their huge disparities in administration routes and dosage. To solve this, we engineered the Fc fragment of human IgG1 into a hexamer nanoparticle and expressed its afucosylated form in Fut8−/− CHO cells, naming it “FcRider.” FcRider is highly soluble with long-term stability, easily produced at high levels equivalent to those of therapeutic antibodies, and is amenable to conventional antibody purification schemes. Most importantly, FcRider possesses endogenous adjuvant activities. Using SWHEL B cell receptor (BCR) transgenic mice, we found that HEL-FcRider induced GL7+ germinal center B cells and HEL-specific IgG. Similarly, immunizing mice with UFO-BG-FcRider, a fusion containing the stabilized human immunodeficiency virus-1 (HIV-1) Env protein as immunogen, promoted somatic hypermutation and generation of long CDR3 of the IgG heavy chains. Intramuscular injection of (Fba + Met6)3-FcRider, a fusion with two peptide epitopes from Candida albicans cell surface, stimulated strong antigen-specific IgG titers. In three different models, we showed that afucosylated FcRider functions as a multivalent immunogen displayer and stimulates antigen-specific B cells without any exogenous adjuvant. As an antibody derivative, afucosylated FcRider could be a novel platform combining vaccines and therapeutic antibodies, integrating active and passive immunizations into single-modality “hybrid immunization” to provide complete and long-lasting protection against infections, and may open new avenues in cancer immunotherapy as well.
First Page
295
Last Page
306
PubMed ID
39381134
Volume
7
Issue
4
Chapter Title
023 - FcRider: a recombinant Fc nanoparticle with endogenous adjuvant activities for hybrid immunization
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Mao, Changchuin; Eberle, Karen; Chen, Xiaojie; Zhou, Yiming; Li, Jun; Xin, Hong; and Gao, Wenda, "FcRider: a recombinant Fc nanoparticle with endogenous adjuvant activities for hybrid immunization" (2024). School of Graduate Studies Faculty Publications. 315.
https://digitalscholar.lsuhsc.edu/sogs_facpubs/315
10.1093/abt/tbae023
Included in
Infectious Disease Commons, Medical Immunology Commons, Medical Microbiology Commons, Virology Commons