Additional file 9 of High glucose-upregulated PD-L1 expression through RAS signaling-driven downregulation of PTRH1 leads to suppression of T cell cytotoxic function in tumor environment

Chenggang Gao, Huazhong University of Science and Technology
Jiaoshun Chen, Huazhong University of Science and Technology
Jianwei Bai, Huazhong University of Science and Technology
Haoxiang Zhang, Huazhong University of Science and Technology
Yanyi Tao, Huazhong University of Science and Technology
Shihong Wu, Huazhong University of Science and Technology
Hehe Li, Huazhong University of Science and Technology
Heshui Wu, Huazhong University of Science and Technology
Qiang Shen, LSU Health Sciences Center - New Orleans
Tao Yin, Huazhong University of Science and Technology

Original Article:

https://doi.org/10.1186/s12967-023-04302-12

Abstract

Additional file 9: Figure S9 A Western blotting analysis of PD-L1 expression in PANC-1 and SW1990 cells following 12 h treatment with AICAR in 5.5 mM glucose medium. B Western blotting analysis of PD-L1 expression in PANC-1 and SW1990 cells following 72 h treatment with KRAS or STAT3 is in 25 mM glucose medium. C Western blotting analysis of PD-L1 expression in PANC-1 and SW1990 cells following 48 h treatment with PI3K-IN-1 in 25 mM glucose medium. The graphs show representative results from three independently repeated experiments. *: p value < 0.05, **: p value < 0.01, ***: p value < 0.001