Additional file 4 of High glucose-upregulated PD-L1 expression through RAS signaling-driven downregulation of PTRH1 leads to suppression of T cell cytotoxic function in tumor environment

Chenggang Gao, Huazhong University of Science and Technology
Jiaoshun Chen, Huazhong University of Science and Technology
Jianwei Bai, Huazhong University of Science and Technology
Haoxiang Zhang, Huazhong University of Science and Technology
Yanyi Tao, Huazhong University of Science and Technology
Shihong Wu, Huazhong University of Science and Technology
Hehe Li, Huazhong University of Science and Technology
Heshui Wu, Huazhong University of Science and Technology
Qiang Shen, LSU Health Sciences Center - New Orleans
Tao Yin, Huazhong University of Science and Technology

Original Article:

https://doi.org/10.1186/s12967-023-04302-7

Abstract

Additional file 4: Figure S4 Single cell RNA-seq data processing of CD8+ T cells. A Processing of identification of CD8+ T cells. B Violin plot depicting the feature and count of genes as well as the percentage of mitochondrial genes. C Correlation analysis of feature genes to detect sequencing depth. D Variogram depicting variable feature genes among cells. E Principal Component Analysis (PCA) of cells. F Dot plot depicting feature genes of each Principal Component. G Heatmap depicting variable feature genes of PCA. H JackStraw Plot of PC1 to 20. I Dot plot depicting expression of markers of T cell subsets of clusters. Single cell RNA-seq data was downloaded from GSA: CRA001160.